Difference between revisions of "Part:BBa K415147:Experience"
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===Applications of BBa_K415147=== | ===Applications of BBa_K415147=== | ||
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| + | '''Assessing p8-Fos incorporation into phage coat''' | ||
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| + | We transformed XL1-Blue cells with BBa_K415147, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-Myc antibody. | ||
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| + | [[Image:1023westernfos.jpg]] | ||
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| + | The purple band at 13 kD represents the p8-Fos fusion, and is evidence of the fusion protein's incorporation into the phage coat. We are currently looking into possible explanations for the fainter band at 15 kD. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 06:04, 25 October 2010
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Please enter
how you used this part and how it worked out.
Applications of BBa_K415147
Assessing p8-Fos incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415147, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-Myc antibody.
The purple band at 13 kD represents the p8-Fos fusion, and is evidence of the fusion protein's incorporation into the phage coat. We are currently looking into possible explanations for the fainter band at 15 kD.
User Reviews
UNIQf45e0dcade1df43e-partinfo-00000000-QINU UNIQf45e0dcade1df43e-partinfo-00000001-QINU