Difference between revisions of "Part:BBa K364308:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Ligand===
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PXR is activated by a large number of endogenous and exogenous chemicals including steroids, antibiotics, antimycotics, bile acids, hyperforin, a constituent of the herbal antidepressant St. John's Wort, and many other herbal compounds.
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PXR is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin.
  
 
===Physiological effets of PXR===
 
===Physiological effets of PXR===
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===Potential applications===
 
===Potential applications===
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ER-DBD can be fused with various LBDs in order to get heterogenous composite Nuclear Receptors. With addition of the LBD's ligand the receptor can bind to Estrogen Receptor Response element and activate downstream gene expression.
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Human PXR LBD can be used to assemble heterogenous Nuclear Receptors. With the addition of PXR ligands, any kind of genes can be activated depending on the fused DBD.
  
 
===Applications of BBa_K364308===
 
===Applications of BBa_K364308===

Latest revision as of 01:41, 26 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Ligand

PXR is activated by a large number of endogenous and exogenous chemicals including steroids, antibiotics, antimycotics, bile acids, hyperforin, a constituent of the herbal antidepressant St. John's Wort, and many other herbal compounds.

PXR is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin.

Physiological effets of PXR

Like other type II nuclear receptors, when activated, it forms a heterodimer with the retinoid X receptor, and binds to hormone response elements on DNA which elicits expression of gene products.

One of the primary targets of PXR activation is the induction of CYP3A4, an important phase I oxidative enzyme that is responsible for the metabolism of many drugs. In addition, PXR up regulates the expression of phase II conjugating enzymes such as glutathione S-transferase and phase III transport uptake and efflux proteins such as OATP2 (SLCO2A1)and MDR1(ABCB1).

Potential applications

Human PXR LBD can be used to assemble heterogenous Nuclear Receptors. With the addition of PXR ligands, any kind of genes can be activated depending on the fused DBD.

Applications of BBa_K364308

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UNIQb65452cdfd877092-partinfo-00000000-QINU UNIQb65452cdfd877092-partinfo-00000001-QINU