Difference between revisions of "Part:BBa K364314:Design"

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===Design Notes===
 
===Design Notes===
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The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25
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The construction of the LBD and pSB1C3 backbone have been done with RFC 25.
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===Source===
 
===Source===
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1. Lindblom TH, Pierce GJ, Sluder AE,"A C. elegans orphan nuclear receptor contributes to xenobiotic resistance"
 
1. Lindblom TH, Pierce GJ, Sluder AE,"A C. elegans orphan nuclear receptor contributes to xenobiotic resistance"
 
[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRT-438BMM3-P-1&_cdi=6243&_user=10&_pii=S0960982201002366&_coverDate=06%2F01%2F2001&_sk=%23TOC%236243%232001%23999889988%23253153%23FLA%23display%23Volume_11,_Issue_11,_Pages_R413-R450,_809-908_(1_June_2001)%23tagged%23Volume%23first%3D11%23Issue%23first%3D11%23date%23(1_June_2001)%23&view=c&_gw=y&wchp=dGLbVlW-zSkzV&md5=e1002ea78d04776b5b9ccc020f6c65e5&ie=/sdarticle.pdf Curr Biol. 2001 Jun 5;11(11):864-8.]
 
[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRT-438BMM3-P-1&_cdi=6243&_user=10&_pii=S0960982201002366&_coverDate=06%2F01%2F2001&_sk=%23TOC%236243%232001%23999889988%23253153%23FLA%23display%23Volume_11,_Issue_11,_Pages_R413-R450,_809-908_(1_June_2001)%23tagged%23Volume%23first%3D11%23Issue%23first%3D11%23date%23(1_June_2001)%23&view=c&_gw=y&wchp=dGLbVlW-zSkzV&md5=e1002ea78d04776b5b9ccc020f6c65e5&ie=/sdarticle.pdf Curr Biol. 2001 Jun 5;11(11):864-8.]
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2. T. H. Lindblom, A. E. Sluder. "nhr-8 is Required for Gut Function and May Respond to the Food Signal". International C. elegans Meeting (1999).

Latest revision as of 18:02, 25 October 2010

NHR-8 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 96


Design Notes

The construction of the LBD and pSB1C3 backbone have been done with RFC 25.

Source

C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-8 placed into pSB1C3 carrier vector.

References

1. Lindblom TH, Pierce GJ, Sluder AE,"A C. elegans orphan nuclear receptor contributes to xenobiotic resistance" [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRT-438BMM3-P-1&_cdi=6243&_user=10&_pii=S0960982201002366&_coverDate=06%2F01%2F2001&_sk=%23TOC%236243%232001%23999889988%23253153%23FLA%23display%23Volume_11,_Issue_11,_Pages_R413-R450,_809-908_(1_June_2001)%23tagged%23Volume%23first%3D11%23Issue%23first%3D11%23date%23(1_June_2001)%23&view=c&_gw=y&wchp=dGLbVlW-zSkzV&md5=e1002ea78d04776b5b9ccc020f6c65e5&ie=/sdarticle.pdf Curr Biol. 2001 Jun 5;11(11):864-8.]

2. T. H. Lindblom, A. E. Sluder. "nhr-8 is Required for Gut Function and May Respond to the Food Signal". International C. elegans Meeting (1999).