Difference between revisions of "Part:BBa K320004"
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− | This BioBrick | + |
This BioBrick consists of an origin of replication for Asaia and E. coli (<partinfo>BBa_K320000</partinfo>) and a resistance to Kanamycin (<partinfo>BBa_P1003</partinfo>) . It was initially designed to be inserted into (<partinfo>BBa_I51020 </partinfo>). |
− | <!-- Add more about the biology of this part here | + | We developed this BioBricks as a tool for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia''] |
+ | |||
+ | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K320004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K320004 SequenceAndFeatures</partinfo> | ||
− | <!-- Uncomment this to enable Functional Parameter display | + | <!-- Uncomment this to enable Functional Parameter display |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K320004 parameters</partinfo> | <partinfo>BBa_K320004 parameters</partinfo> | ||
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Latest revision as of 16:23, 27 October 2010
Origin for Asaia and resistance to Kanamycin
This BioBrick consists of an origin of replication for Asaia and E. coli (BBa_K320000) and a resistance to Kanamycin (BBa_P1003) . It was initially designed to be inserted into (BBa_I51020).
We developed this BioBricks as a tool for our new chassis [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1166
Illegal AgeI site found at 1326 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4