Difference between revisions of "Part:BBa K320002:Design"

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===Design Notes===
 
===Design Notes===
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The sequence was syntethis from the E. coli promoter consensus sequence.
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The sequence was artificially made from two primers containing the ''E. coli'' consensus sequences for promoter and RBS with an appropriate spacing.
  
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===Source===
 
  
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The source is the article [http://www.pnas.org/content/104/21/9047.long [1]].
 
  
 
===References===
 
===References===
  
 
# [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]
 
# [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]

Latest revision as of 12:33, 27 October 2010

Strong promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was artificially made from two primers containing the E. coli consensus sequences for promoter and RBS with an appropriate spacing.


References

  1. [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]