Difference between revisions of "Part:BBa K320000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This | + | This part was obtained through PCR. |
===Source=== | ===Source=== | ||
− | This | + | This BioBrick was obtained from pHM2-GFP [http://www.pnas.org/content/104/21/9047.long [1]]a strand of Asaia containing a plasmid with a GFP-gene. This plasmid was created by the team of Prof. Guido Favia. |
===References=== | ===References=== |
Latest revision as of 12:24, 27 October 2010
Asaia replication origin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1160
Illegal AgeI site found at 1320 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was obtained through PCR.
Source
This BioBrick was obtained from pHM2-GFP [http://www.pnas.org/content/104/21/9047.long [1]]a strand of Asaia containing a plasmid with a GFP-gene. This plasmid was created by the team of Prof. Guido Favia.
References
- [http://www.pnas.org/content/104/21/9047.long Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector]
- [http://aem.asm.org/cgi/content/abstract/68/5/2619 Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens]