Difference between revisions of "Part:BBa K320000"

Latest revision as of 21:13, 27 October 2010

Asaia replication origin

 This biobrick was obtained by a PCR on the pHM2-GFP plasmid (See: [http://www.pnas.org/content/104/21/9047.long Bacteria of the genus Asaia stably associate with Anopheles stephensii, an Asian malarial mosquito vector]). The Asaia that contained this plasmid was provided to us by the team of Prof. Guido Favia (Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy).

Asaia is a gram negative bacterium, member of the family of acetic acid bacteria in the alpha-Proteobacteria. [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia] is naturally present in the gut of at least four mosquito species which are responsible for the transmission of Malaria (Anopheles stephensii, Anopheles gambiae, Aedes aegypti and Aedes albopictus).

 This origin of replication works in Asaia as well as E. coli. No other bacteria were tested but [http://www.mobitec.com/int/products/bio/04_vector_sys/pbbr122.html the literature] shows that it works in many gram-negative bacteria.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1160
Illegal AgeI site found at 1320
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K320000 short</partinfo>
-This sequence was obtain by making a PCR form the GFP plasmid of a straight of Asaia containing this plasmid. This strain has been provided to us by the team of Prof. Guido Favia from Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy.
+ This biobrick was obtained by a PCR on the pHM2-GFP plasmid (See: [http://www.pnas.org/content/104/21/9047.long Bacteria of the genus Asaia stably associate with Anopheles stephensii, an Asian malarial mosquito vector]). The Asaia that contained this plasmid was provided to us by the team of Prof. Guido Favia (Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy).
-This origin of replication works is Asaia and E.Choli.
+Asaia is a gram negative bacterium, member of the family of acetic acid bacteria in the alpha-Proteobacteria.
+[http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia] is naturally present in the gut of at least four mosquito species which are responsible for the transmission of Malaria (''Anopheles stephensii'', ''Anopheles gambiae'', ''Aedes aegypti'' and ''Aedes albopictus'').
+ This origin of replication works in Asaia as well as ''E. coli''. No other bacteria were tested but  [http://www.mobitec.com/int/products/bio/04_vector_sys/pbbr122.html  the literature] shows that it works in many gram-negative bacteria.
+===Sequence and Features===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K320000 SequenceAndFeatures</partinfo>