Difference between revisions of "Part:BBa K313027"

Latest revision as of 03:48, 28 October 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1006.

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.

The difference between this part and BBa K313018 is the number of the terminator.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 619
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 305
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K313027 short</partinfo>
-This is a device that aims to measure the leakiness of terminator BBa_B1006.
+This is a device that aims to measure the leakiness of a terminator BBa_B1006.
+If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
+With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
+The difference between this part and BBa K313018 is the number of the terminator.
+Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.