Difference between revisions of "Part:BBa K313006"
 
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<partinfo>BBa_K313006 short</partinfo>
 
<partinfo>BBa_K313006 short</partinfo>
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cDNA of MS2phage (XbaI site is removed)
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This part is a cDNA of the MS2 phage genome. (XbaI site is removed.)
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We succeeded in making this cDNA from the original RNA of the phage by RT-PCR.
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This cDNA includes four coding regions, one of which is transcribed with the use of the frameshift.
 
This cDNA includes four coding regions, one of which is transcribed with the use of the frameshift.
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It also includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis.
 
  
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The sequence of the MS2 phage genome originally includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis.
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2  Phage MS2] assay page.
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 02:49, 28 October 2010

MS2 phage cDNA (XbaI site is removed)

This part is a cDNA of the MS2 phage genome. (XbaI site is removed.)

We succeeded in making this cDNA from the original RNA of the phage by RT-PCR.

This cDNA includes four coding regions, one of which is transcribed with the use of the frameshift.

The sequence of the MS2 phage genome originally includes two EcoRI and one XbaI site. We removed the XbaI site by site-directed mutagenesis.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2 Phage MS2] assay page.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal BamHI site found at 2057
    Illegal XhoI site found at 1048
    Illegal XhoI site found at 3214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 1733
    Illegal NgoMIV site found at 2437
    Illegal NgoMIV site found at 3309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1665
    Illegal BsaI.rc site found at 163
    Illegal BsaI.rc site found at 2257