Difference between revisions of "Part:BBa K316021"

Latest revision as of 23:54, 27 October 2010

Spectinomycin resistance with promoter for B.subtilis

For reliable expression of spectinomycin resistance gene (Aad9 BBa_K143031) in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG BBa_K143053 were used. A double terminator BBa_B0015 ensures there is minimal read-through.

Figure I. Spectinomycin resistance cassette.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 611

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K316021 short</partinfo>
-For reliable expression in B. subtilis the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were combined with spectinomycin resistance gene with terminator <bbpart>BBa_J143065</bbpart>.
+For reliable expression of spectinomycin resistance gene (Aad9 <bbpart>BBa_K143031</bbpart>)  in B. subtilis, the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were used. A double terminator <bbpart>BBa_B0015</bbpart> ensures there is minimal read-through.
+[[Image:SpecCasette.PNG|center|500px]]
+'''Figure I.''' Spectinomycin resistance cassette.