Difference between revisions of "Part:BBa K325108"

 
 
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__NOTOC__
 
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<partinfo>BBa_K325108 short</partinfo>
 
<partinfo>BBa_K325108 short</partinfo>
  
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This part is a translational unit for a mutant of the luciferase from the North American firefly (P. pyralis). It is under the control of an Arabinose induced promoter (pBAD). D-Luciferin has to be added to obtain light output.
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EPIC stands for '''Enhanced Photon Initiating Complex'''. It is described in [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Fujii et al. 2007] as having a 10 times higher substrate affinity and luminescence output compared to wildtype.
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The part is codon optimised for expression in ''E.coli''.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:50, 28 October 2010

EPIC Firefly Luciferase (under pBAD)
P. Pyralis
(E. coli optimised)


This part is a translational unit for a mutant of the luciferase from the North American firefly (P. pyralis). It is under the control of an Arabinose induced promoter (pBAD). D-Luciferin has to be added to obtain light output.

EPIC stands for Enhanced Photon Initiating Complex. It is described in [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Fujii et al. 2007] as having a 10 times higher substrate affinity and luminescence output compared to wildtype.

The part is codon optimised for expression in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2845
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2549