Difference between revisions of "Part:BBa K415300"
 
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<partinfo>BBa_K415300 short</partinfo>
 
<partinfo>BBa_K415300 short</partinfo>
  
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From <i>Construction of a genetic toggle switch in E. Coli.</i> Collins Toggle, Advanced Biotechnology, BU.
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From <i>Programmable cells: Interfacing natural and engineered gene networks</i>, Kobayashi, H. et. al, PNAS. [[Image:CollinsToggle.png|frame|150px|right|The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.]]
  
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The [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html|Collins UV Toggle] (2000) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.
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The [http://www.pnas.org/content/101/22/8414.full Collins/Kobayashi UV Toggle] (2004) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.<br><br>
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; width: 200px; border-style: solid; border-color: #000; width: 500px; display: inline-block;"
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!UV Power Modulation
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This part has since been improved by the '''[http://2010.igem.org/Team:MIT 2010 MIT iGEM Team]'''. The new, low power toggle [[Part:BBa_K415301]] requires less UV power to switch states, and thus more cells survive the exposure process.
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The following data was taken from cells co-transformed with pTSMa and [[Part:BBa_K415023]].
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{|style="border-width: 1px; width: 200px; border-style: solid; border-color: #000; width: 700px; text-align:center;"
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!colspan="6"|
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== UV Power Modulation ==
 
|-
 
|-
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|Photos
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|colspan="2"|'''Photos'''
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| <i>Image</i>
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(in J/m^2, ignore W)
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|colspan="4"|[[Image:Igem_uv_prelim.png]]
 
|-
 
|-
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|FACS
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|colspan="2"|'''FACS'''
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| <i>Image</i>
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(in J/m^2)
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|}
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|colspan="1"|[[Image:0.png|110px]]
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{|style=" display:inline; font-size: x-small;" align=right
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|colspan="1"|[[Image:5.png|110px]]
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|colspan="1"|[[Image:20.png|110px]]
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|colspan="1"|[[Image:40.png|110px]]
 
|-
 
|-
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|[[Image:CollinsToggle.png|150px|center]]
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|colspan="2"|  
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|-
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|colspan="1"|0
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|The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.
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|colspan="1"|5
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|}<br><br><br><br>
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|colspan="1"|20
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|colspan="1"|40
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|}
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Latest revision as of 22:39, 25 October 2010

Collins UV Toggle - Ptrc:cI:tt:Plambda:lacI:tt

From Programmable cells: Interfacing natural and engineered gene networks, Kobayashi, H. et. al, PNAS.
The original pTSMa plasmid from the Collins paper. The parts have since been biobricked into pSB1C3.

The [http://www.pnas.org/content/101/22/8414.full Collins/Kobayashi UV Toggle] (2004) biobricked. It is a bistable toggle (on or off state) and switching to state 1 is induced by UV exposure and to state 2 is IPTG. If the toggle is set with IPTG, the cells will express cI which will inhibit Plambda. If this is exposed to certain levels of UV (see power modulations) cI is cleaved by Rec-A (a UV induced enzyme) and lacI expression begins and inhibits Ptrc.

This part has since been improved by the [http://2010.igem.org/Team:MIT 2010 MIT iGEM Team]. The new, low power toggle Part:BBa_K415301 requires less UV power to switch states, and thus more cells survive the exposure process.

The following data was taken from cells co-transformed with pTSMa and Part:BBa_K415023.

UV Power Modulation

Photos

(in J/m^2, ignore W)

Igem uv prelim.png
FACS

(in J/m^2)

0.png 5.png 20.png 40.png
0 5 20 40




Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]