Difference between revisions of "Part:BBa K330002:Design"

(New page: __NOTOC__ <partinfo>BBa_K330002 short</partinfo> <partinfo>BBa_K330002 SequenceAndFeatures</partinfo>)
 
(→‎Design notes)
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
  
 
<partinfo>BBa_K330002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K330002 SequenceAndFeatures</partinfo>
 +
 +
 +
 +
'''Design notes'''
 +
 +
 +
 +
The gusA coding sequence was amplified by PCR with plasmid PBI121 (Kmr) as the template. Restriction enzyme cutting sites EcoRI, XbaI, SpeI and PstI were added in the overhangs of two primers following the standard assembly requirements.
 +
 +
 +
Below are primer sequences for the PCR:
 +
 +
gusA F      GGTGCCCGGGATGTTACGTCCTGTAGAAACCC
 +
 +
gusA R      ATACCTGCAGACTAGTTTATTGTTTGCCTCCCTGC

Latest revision as of 16:10, 23 October 2010

gusA reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design notes


The gusA coding sequence was amplified by PCR with plasmid PBI121 (Kmr) as the template. Restriction enzyme cutting sites EcoRI, XbaI, SpeI and PstI were added in the overhangs of two primers following the standard assembly requirements.


Below are primer sequences for the PCR:

gusA F GGTGCCCGGGATGTTACGTCCTGTAGAAACCC

gusA R ATACCTGCAGACTAGTTTATTGTTTGCCTCCCTGC