Difference between revisions of "Part:BBa K364308"
 
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PXR is a xenobiotic-activated member of the nuclear receptor superfamily of transcription factors. PXR is involved in transcriptional induction of hepatic xenobiotic-catabolizing cytochrome 3A enzymes, playing a fundamental role in protecting body tissues from toxic bile acids. PXR is almost exlcusively expressed in the gastrointestinal system (stomach, duodenum, jejunum, ileum, colon and gall bladder) and liver, with lower levels in the kidney and ovary. PXR dysfunction is associated with immune disorders (sclerosing cholangitis, inflammatory bowel disease) and biliary primary cirrhosis.
 
PXR is a xenobiotic-activated member of the nuclear receptor superfamily of transcription factors. PXR is involved in transcriptional induction of hepatic xenobiotic-catabolizing cytochrome 3A enzymes, playing a fundamental role in protecting body tissues from toxic bile acids. PXR is almost exlcusively expressed in the gastrointestinal system (stomach, duodenum, jejunum, ileum, colon and gall bladder) and liver, with lower levels in the kidney and ovary. PXR dysfunction is associated with immune disorders (sclerosing cholangitis, inflammatory bowel disease) and biliary primary cirrhosis.
  
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More on PXR here: [http://en.wikipedia.org/wiki/Pregnane_X_receptor].
  
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PXR Uniprot: [http://www.uniprot.org/uniprot/O75469].
  
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View the PXR LBD crystal structure here: [http://www.pdb.org/pdb/explore/jmol.do?structureId=1SKX&bionumber=1].
  
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[[Image:PXR-LBD with Rifampicin.jpg|800px|thumb|center| Structure of human Pregnan X receptor ligand binding domain in complex with the macrolide antibiotic Rifampicin.]]
  
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Expression
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Description of the PXR LBD crystal structure with reference here: [http://mend.endojournals.org/cgi/content/full/19/5/1125].
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[[Image:PXR LBD with Rifampicin.jpg]]  
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[[Image:PXRLBD.jpg|800px|thumb|center|Picture of gel electrophoresis: PXR LBD in pSB1C3 resulting an insert of 807 bp.]]
  
 
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Latest revision as of 21:50, 25 October 2010

Human PXR LBD

PXR is a xenobiotic-activated member of the nuclear receptor superfamily of transcription factors. PXR is involved in transcriptional induction of hepatic xenobiotic-catabolizing cytochrome 3A enzymes, playing a fundamental role in protecting body tissues from toxic bile acids. PXR is almost exlcusively expressed in the gastrointestinal system (stomach, duodenum, jejunum, ileum, colon and gall bladder) and liver, with lower levels in the kidney and ovary. PXR dysfunction is associated with immune disorders (sclerosing cholangitis, inflammatory bowel disease) and biliary primary cirrhosis.

More on PXR here: [http://en.wikipedia.org/wiki/Pregnane_X_receptor].

PXR Uniprot: [http://www.uniprot.org/uniprot/O75469].

View the PXR LBD crystal structure here: [http://www.pdb.org/pdb/explore/jmol.do?structureId=1SKX&bionumber=1].

Structure of human Pregnan X receptor ligand binding domain in complex with the macrolide antibiotic Rifampicin.

Description of the PXR LBD crystal structure with reference here: [http://mend.endojournals.org/cgi/content/full/19/5/1125].

Picture of gel electrophoresis: PXR LBD in pSB1C3 resulting an insert of 807 bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 134
    Illegal BglII site found at 245
    Illegal BamHI site found at 668
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]