Difference between revisions of "Part:BBa K325219:Stability"
(→Data) |
(→Data) |
||
Line 10: | Line 10: | ||
[[Image:Phhistogram.png|thumb|569px|center|'''Figure 1 - Maximum light output within 5 hours of D-luciferin injection at different pH values. These values are the mean of 3 readings. The corresponding error bars represent an interval of twice the standard deviation across the 3 data points centred around the mean value. ''']] | [[Image:Phhistogram.png|thumb|569px|center|'''Figure 1 - Maximum light output within 5 hours of D-luciferin injection at different pH values. These values are the mean of 3 readings. The corresponding error bars represent an interval of twice the standard deviation across the 3 data points centred around the mean value. ''']] | ||
− | [[Image: | + | [[Image:Phtimecourse.png|thumb|569px|center|'''Figure 2 - Evolution of light output at different values of pH. Measurements are taken every 20 min. These values are the mean of 3 readings. The corresponding error bars represent an interval of twice the standard deviation across the 3 data points centred around the mean value. ''']] |
− | + | ||
<center> | <center> | ||
{|{{Table}} | {|{{Table}} |
Latest revision as of 17:10, 23 October 2010
|
|
Description
Both the intensity and the spectrum emitted by the luciferase-luciferin reaction has been shown to be higly dependent on the pH of the medium. The main characterisation experiments have been performed in LB Broth at pH 7, so in order to assess this effect cultures with LB and a citrate buffer were prepared (pH = 5.3, pH 6.1 and pH = 7) This page describes the results of these experiments. We used a [http://www.bmglabtech.com/products/microplate-reader/instruments.cfm?product_id=2 FLUOstar OPTIMA] microplate reader to quantify the light output. Protocols and plate reader settings used are given below.
Data
Data | Notes | Date Uploaded |
---|---|---|
Media:BBa_K325219pheffect.xls | Raw data from experiment | 21/10/2010 |
Protocol
- The protocol can be found as [http://2010.igem.org/Team:Cambridge/Notebook/Week11 Experiment 110] on the [http://2010.igem.org/Team:Cambridge Cambridge iGEM 2010 Website].
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3
References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.
[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.