Difference between revisions of "Part:BBa K313021"

 
 
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<partinfo>BBa_K313021 short</partinfo>
 
<partinfo>BBa_K313021 short</partinfo>
  
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This is a device that aims to measure the leakiness of the terminator BBa_B0017
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This is a device that aims to measure the leakiness of a terminator BBa_B0017.
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If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
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With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
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Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw  Terminator leak] assay page.
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<!-- Add more about the biology of this part here
 
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Latest revision as of 03:40, 28 October 2010

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B0017.

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
  • 1000
    COMPATIBLE WITH RFC[1000]