Difference between revisions of "Part:BBa K346011"

 
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<partinfo>BBa_K346011 short</partinfo>
 
<partinfo>BBa_K346011 short</partinfo>
  
This part is composed of constutitive promoter BBa_J23109 and transcription factor MerR. It can regulate PmerT to represent a dose-response manner to response to Hg(II) concentration.
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We used the structure along with PmerT-RBS-GFP to see under the constant MerR concentration level how GFP expression vary according to the Hg(II) concentration.  
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This part is composed of the constutitive promoter BBa_J23109 and a transcription factor merR. MerR can activate PmerT as a dimer in the presence of Hg(II) in a dose-response manner. We used the structure along with PmerT-RBS-GFP(BBa_K346096) to see how GFP expression vary in certain concentration of MerR when indcued by different concentraton of the Hg(II). For more information about merR, please click [https://parts.igem.org/Part:BBa_K346001 RBS-merR(BBa_K346001)]
  
 
[[Image:4.jpg]]
 
[[Image:4.jpg]]

Latest revision as of 20:47, 27 October 2010

Constutitive promoter(BBa_J23109)+RBS+MerR(BBa_K346001)


This part is composed of the constutitive promoter BBa_J23109 and a transcription factor merR. MerR can activate PmerT as a dimer in the presence of Hg(II) in a dose-response manner. We used the structure along with PmerT-RBS-GFP(BBa_K346096) to see how GFP expression vary in certain concentration of MerR when indcued by different concentraton of the Hg(II). For more information about merR, please click RBS-merR(BBa_K346001)

4.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]