Difference between revisions of "Part:BBa K337031"

 
 
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<partinfo>BBa_K337031 short</partinfo>
 
<partinfo>BBa_K337031 short</partinfo>
  
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RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by twice Tet operator.  
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RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by a double Tet operator. This part can be used for regulation the expressio of any GOI via the Tet System (BB-2, RFC12).
  
 
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Latest revision as of 09:17, 27 October 2010

BGH(rc)/shRNA6(rc)/RSV(rc)/CMV_TetO2(fw)

RSV promoter driving a designed, synthetic shRNA-like miRNA followed by the BGH terminator. Those elements are all rc, CMV promoter near RSV drives in the opposite direction (fw) followed by a double Tet operator. This part can be used for regulation the expressio of any GOI via the Tet System (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1659
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]