Difference between revisions of "Part:BBa K349119:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K349119=== | ===Applications of BBa_K349119=== | ||
| + | Digestion at the BsaI cut sites inside of the prefix and suffix will create an A overhang at the 5' end and a B overhang at the 3' end. These ends are compatible with the BioBytes 2.0 assembly method. | ||
===User Reviews=== | ===User Reviews=== | ||
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| + | Karina Arnesen- Alberta iGEM team 2010- This part does not work, when incorporated with a promoter, no fluorescence is observed. I noticed that the design of the part incorporates a frame shift mutation, likely causing loss of function of the protein. To fix, either at a C between bases 14 and 15, this will make the part compatible with BioBytes 2.0, or incorporate a T between bases 13 and 14 will reconstitute the wildtype sequence as per the Geneback accession number: EU024648. | ||
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<!-- DON'T DELETE --><partinfo>BBa_K349119 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K349119 StartReviews</partinfo> | ||
<!-- Template for a user review | <!-- Template for a user review | ||
Latest revision as of 18:11, 11 August 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K349119
Digestion at the BsaI cut sites inside of the prefix and suffix will create an A overhang at the 5' end and a B overhang at the 3' end. These ends are compatible with the BioBytes 2.0 assembly method.
User Reviews
Karina Arnesen- Alberta iGEM team 2010- This part does not work, when incorporated with a promoter, no fluorescence is observed. I noticed that the design of the part incorporates a frame shift mutation, likely causing loss of function of the protein. To fix, either at a C between bases 14 and 15, this will make the part compatible with BioBytes 2.0, or incorporate a T between bases 13 and 14 will reconstitute the wildtype sequence as per the Geneback accession number: EU024648.
UNIQd2ac65a9bdf161d7-partinfo-00000000-QINU UNIQd2ac65a9bdf161d7-partinfo-00000001-QINU