Difference between revisions of "Part:BBa K337011"

Latest revision as of 09:43, 27 October 2010

hsa-miR-221 Binding site pattern (2BS)

hsa-miR-221 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K337011 short</partinfo>
-hsa-miR-221 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites.
+hsa-miR-221 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).
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