Difference between revisions of "Part:BBa K337011"
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| − | + | hsa-miR-221 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12). | |
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Latest revision as of 09:43, 27 October 2010
hsa-miR-221 Binding site pattern (2BS)
hsa-miR-221 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]