Difference between revisions of "Part:BBa K337005"

 
 
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<partinfo>BBa_K337005 short</partinfo>
 
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hsa-miR-122 binding site pattern, consisting of 2 perfect matching binding sites. The binding sites are seperated by a sequence of 40 base pairs, which are innert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to standard restriction sites.  
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hsa-miR-122 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).  
  
 
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Latest revision as of 09:35, 27 October 2010

hsa-miR-122 Binding site pattern (2BS - additional 10bp Spacer)

hsa-miR-122 binding site pattern, consisting of 2 perfect matching binding sites (BS). The BS are seperated by a sequence of 40 bp, which are inert in terms of miRNA binding. Additionally, first 15bp and last 15bp of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]