Difference between revisions of "Part:BBa K337003"
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<partinfo>BBa_K337003 short</partinfo> | <partinfo>BBa_K337003 short</partinfo> | ||
| − | hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are | + | hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12). |
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Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching: | Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching: | ||
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| − | 2nd: CAAACACCATTGTCA-ACTCCA | + | :1st: CAAACACCATTGTCACACTC-A |
| − | perfect: CAAACACCATTGTCACACTCCA | + | :perfect: CAAACACCATTGTCACACTCCA |
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| + | :2nd: CAAACACCATTGTCA-ACTCCA | ||
| + | :perfect: CAAACACCATTGTCACACTCCA | ||
Latest revision as of 09:34, 27 October 2010
hsa-miR-122 Binding site pattern (4BS)
hsa-miR-122 binding site pattern, consisting of 4 binding sites (BS). The BS are seperated by sequences of 30 base pairs, which are inert in terms of miRNA binding. Additionally, half of the separation sequence is either in front or the end of the whole pattern, to be unconnected to the standard restriction sites (BB-2, RFC12).
Mutations showed up for BS 1 and 2, where BS 3 and 4 are perfectly matching:
- 1st: CAAACACCATTGTCACACTC-A
- perfect: CAAACACCATTGTCACACTCCA
- 2nd: CAAACACCATTGTCA-ACTCCA
- perfect: CAAACACCATTGTCACACTCCA
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]