Difference between revisions of "Part:BBa K317028:Design"

 
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===Design Notes===
 
===Design Notes===
 
The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication
 
The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication
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[[Image:Phototaxis Device.png|400px]]
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[[Image:Primer sets.png|400px]]
  
  
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Primer 13 and 14 were used.
  
 
===Source===
 
===Source===

Latest revision as of 20:59, 27 October 2010

Phototaxis Device (Atractant)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 1850
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 134
    Illegal NgoMIV site found at 392
    Illegal AgeI site found at 1652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1088
    Illegal BsaI.rc site found at 1367
    Illegal SapI site found at 862
    Illegal SapI.rc site found at 1868


Design Notes

The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication

Phototaxis Device.png Primer sets.png


Primer 13 and 14 were used.

Source

Natronomonas pharaonis (Obtained from National Institute of Technology and Evaluation(NBRC). (NBRC 14720T)) Salmonella Typhimurium


References