Difference between revisions of "Part:BBa K398001"

Latest revision as of 00:34, 28 October 2010

Medium-chain alkane 1-monooxygenase (Gordonia sp. TF6)

This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with Rubredoxin 3, Rubredoxin 4 and Rubredoxin Reductase it forms the minimal component alkane hydroxylase system. For more information see the references below.

See BBa_K398014 for the characterized protein generator.

[1] Fujii, T., et al., Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6. Bioscience Biotechnology and Biochemistry, 2004. 68(10): p. 2171-2177.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K398001 short</partinfo>
-An alkane 1-monooxygenase from Gordonia sp. TF6. It belongs to the non-haem di-iron family of monooxygenases and is a integral cytoplasmic membrane enzyme. This is the catalytic component of the AH system and as such oxidizes (cyclo)alkanes to their respective (cyclo)alkanols by transferring one oxygen atom from molecular oxygen to the alkane.
+This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with [https://parts.igem.org/Part:BBa_K398002 Rubredoxin 3], [https://parts.igem.org/Part:BBa_K398003 Rubredoxin 4] and [https://parts.igem.org/Part:BBa_K398004 Rubredoxin Reductase] it forms the minimal component alkane hydroxylase system. For more information see the references below.
+See [https://parts.igem.org/Part:BBa_K398014 BBa_K398014] for the characterized protein generator.
+[1] Fujii, T., et al., <i>Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6.</i> Bioscience Biotechnology and Biochemistry, 2004. <b>68</b>(10): p. 2171-2177.
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