Difference between revisions of "Part:BBa K412002:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K412002=== | ===Applications of BBa_K412002=== | ||
| + | [[Image:LacI+RFP, PyeaR+GFP.jpg | 650 px | center]] | ||
| + | *All images were taken with Olympus IX81 automated inverted microscope specially equipped for live cell imaging. All microscope images were taken from live DH5α E.coli cells. The filter sets we used are: 545/30x (excitation) and 620/60m (emission) filters for DsRed, 470/40x (excitation) and 525/50m (emission) for GFP. Data collection and processing was performed by the SlideBook software. | ||
| + | *The data was taken using cells that were grown overnight in 2mL of LB broth that had 50ug/mL of the appropriate antibiotic at 37C and 220rpm. This was used in a 1:50 dilution. Each sample had 40uL from the broth grown overnight and 2mL of the LB broth with the appropriate antibiotic. These were allowed to grow until a predetermined OD. Then the contaminants were added and the cells were allowed to grow for another 2 hours, before the images were taken. | ||
| + | *The images show that the PyeaR promoter does not work in this part. The two right images is the sample incubated in 500uM potassium nitrite, and the two left images is the negative control. The LacI+RFP is constantly on, and the PyeaR+GFP does not respond. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 02:19, 28 October 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K412002
- All images were taken with Olympus IX81 automated inverted microscope specially equipped for live cell imaging. All microscope images were taken from live DH5α E.coli cells. The filter sets we used are: 545/30x (excitation) and 620/60m (emission) filters for DsRed, 470/40x (excitation) and 525/50m (emission) for GFP. Data collection and processing was performed by the SlideBook software.
- The data was taken using cells that were grown overnight in 2mL of LB broth that had 50ug/mL of the appropriate antibiotic at 37C and 220rpm. This was used in a 1:50 dilution. Each sample had 40uL from the broth grown overnight and 2mL of the LB broth with the appropriate antibiotic. These were allowed to grow until a predetermined OD. Then the contaminants were added and the cells were allowed to grow for another 2 hours, before the images were taken.
- The images show that the PyeaR promoter does not work in this part. The two right images is the sample incubated in 500uM potassium nitrite, and the two left images is the negative control. The LacI+RFP is constantly on, and the PyeaR+GFP does not respond.
User Reviews
UNIQ8dcaf7e45e15af62-partinfo-00000000-QINU UNIQ8dcaf7e45e15af62-partinfo-00000001-QINU