Difference between revisions of "Part:BBa K364323:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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===Physiological function of NHR-31===
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The nhr-31 activity is required for proper growth, development, and function of the excretory cell. In regulating excretory cell development, NHR-31 appears to function by controlling the expression of genes encoding subunits of the vacuolar ATPase.
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===Potential applications of GAL4-NHR-31===
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By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).
  
 
===Applications of BBa_K364323===
 
===Applications of BBa_K364323===
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[[Image:pCDNA3.1-Gal4-NHR31.gif|800px|thumb|center|Picture of gel electrophoresis: Gal4-NHR31 in pCDNA3.1 resulting an insert of 974 bp.]]
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[[Image:Team Debrecen WB pCDNA-Gal4-NHR31.jpg|800px|thumb|center|Picture of Western blot: Gal4-NHR31 composite construct is highly expressed after transferring it into pCDNA3.1]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 17:39, 26 October 2010


Physiological function of NHR-31

The nhr-31 activity is required for proper growth, development, and function of the excretory cell. In regulating excretory cell development, NHR-31 appears to function by controlling the expression of genes encoding subunits of the vacuolar ATPase.

Potential applications of GAL4-NHR-31

By the addition of lipid ligands(unknown), the created system activates the expression of any kind of genes fused to UAS(upstream activation seqence)previously. Also can be used as a lipid sensor by activating reporter genes (e.g. Luciferase).

Applications of BBa_K364323

Picture of gel electrophoresis: Gal4-NHR31 in pCDNA3.1 resulting an insert of 974 bp.
Picture of Western blot: Gal4-NHR31 composite construct is highly expressed after transferring it into pCDNA3.1

User Reviews

UNIQca564353180fc56a-partinfo-00000000-QINU UNIQca564353180fc56a-partinfo-00000001-QINU