Difference between revisions of "Part:BBa K323066:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
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We used this part in combination with parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K323132 BBa_K323132] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K323135 BBa_K323135] and showed that this DNA program leads to higher yields of violacein production. [https://parts.igem.org/Part:BBa_K323132:Experience Check results].
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===Applications of BBa_K323066===
 
===Applications of BBa_K323066===
  
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== '''Experience by 2012 iGEM NCTU_FORMOSA''' ==
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The design of our biobricks([[Part:BBa_K887000]]) has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use the DNA program ([[Part:BBa_K323066]])<br>
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However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site.
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[[image:DDpicture1.jpg|700px]]<br>
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So, we designed a complementary primer to insert the lost base pair. After PCR of point mutation, we sequence it again.<br>
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And the outcome is below
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[[image:DDpicture2.jpg|700px]]<br>
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As a result, we successfully correct the lost G base at the zif268 binding site.
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More information, please refer to [[Part:BBa_K887011]]
 
===User Reviews===
 
===User Reviews===
 
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<!-- DON'T DELETE --><partinfo>BBa_K323066 StartReviews</partinfo>

Latest revision as of 05:11, 3 October 2012


We used this part in combination with parts BBa_K323132 and BBa_K323135 and showed that this DNA program leads to higher yields of violacein production. Check results.

Applications of BBa_K323066

Experience by 2012 iGEM NCTU_FORMOSA

The design of our biobricks(Part:BBa_K887000) has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use the DNA program (Part:BBa_K323066)

However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site.

DDpicture1.jpg


So, we designed a complementary primer to insert the lost base pair. After PCR of point mutation, we sequence it again.

And the outcome is below

DDpicture2.jpg

As a result, we successfully correct the lost G base at the zif268 binding site.

More information, please refer to Part:BBa_K887011

User Reviews

UNIQdb8d11da103d2884-partinfo-00000000-QINU UNIQdb8d11da103d2884-partinfo-00000001-QINU