Difference between revisions of "Part:BBa K317998"
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− | Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of β-D-(β-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, | + | Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of β-D-(β-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, rhamnolipid indicating emulsification ability will be produced. This part includes 1 PstI site. |
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Latest revision as of 01:32, 22 October 2021
RhlA
Pseudomonas aeruginosa produces rhamnose-containing glycolipid biosurfactants called rhamnolipids. Rhamnolipid is composed of β-D-(β-D-hydroxyalkanoyloxy)alkanoic acids(HAA) derivatized with one- or two-rhamnose sugars.In rhamnolipid biosynthesis, RhlA is required for the formation of the HAA portion of the molecule and RhlB plays a role of the rhamnosyl transferase. When RhlA and RhlB are expressed in E.coli BL21(DE3) strain, rhamnolipid indicating emulsification ability will be produced. This part includes 1 PstI site.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 720
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 720
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 720
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 720
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478