Difference between revisions of "Part:BBa K358019:Design"
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===Design Notes=== | ===Design Notes=== | ||
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Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used low copy plasmid, pSB4K5, and then transformed it to KRX, not Top 10. | Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used low copy plasmid, pSB4K5, and then transformed it to KRX, not Top 10. | ||
It has lacI in the genome. See KRX genotype here, [http://www.promega.com/pnotes/94/14410_27/14410_27.pdf]. | It has lacI in the genome. See KRX genotype here, [http://www.promega.com/pnotes/94/14410_27/14410_27.pdf]. | ||
Latest revision as of 11:49, 23 October 2010
Lysis cassette regulated by lacP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used low copy plasmid, pSB4K5, and then transformed it to KRX, not Top 10. It has lacI in the genome. See KRX genotype here, [http://www.promega.com/pnotes/94/14410_27/14410_27.pdf].
Source
biobrick[BBa_R0011], lambda phage DNA[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf].