Difference between revisions of "Part:BBa K358019:Design"

 
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<partinfo>BBa_K358019 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used KRX, not Top 10.  This part is toxic to E.coli.
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[[image:K358019.jpg]]
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Because of the lysis cassette, lactose promoter must be repressed when transform it.  So, we used low copy plasmid, pSB4K5, and then transformed it to KRX, not Top 10.
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It has lacI in the genome.  See KRX genotype here, [http://www.promega.com/pnotes/94/14410_27/14410_27.pdf].
  
 
===Source===
 
===Source===
  
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biobrick, lambda phage DNA
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biobrick[BBa_R0011], lambda phage DNA[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf].
  
 
===References===
 
===References===

Latest revision as of 11:49, 23 October 2010

Lysis cassette regulated by lacP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

K358019.jpg

Because of the lysis cassette, lactose promoter must be repressed when transform it. So, we used low copy plasmid, pSB4K5, and then transformed it to KRX, not Top 10. It has lacI in the genome. See KRX genotype here, [http://www.promega.com/pnotes/94/14410_27/14410_27.pdf].

Source

biobrick[BBa_R0011], lambda phage DNA[http://catalog.takara-bio.co.jp/PDFFiles/3403_DS_j.pdf].

References