Difference between revisions of "Part:BBa K314200:Design"

 
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<partinfo>BBa_K314200 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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mention something about promoter needs to have no leaky expression for succesful cloning
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When planning on expressing Tse2 a promoter with no or very little leaky expression must be used. We were only able to obtain non-functioning mutants of the protein when we attempted to biobrick Tse2 downstream from [https://parts.igem.org/Part:BBa_F2620 F2620], probably due to leaky expression of the Tse2 toxin from this promoter.
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===Source===
 
===Source===
  
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pseudomonas aeruginosa
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''Pseudomonas aeruginosa''
  
 
===References===
 
===References===
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Cell Host Microbe.2010 Jan 21;7(1):25-37 PMID: 20114026

Latest revision as of 22:43, 21 October 2010

Toxin Tse2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When planning on expressing Tse2 a promoter with no or very little leaky expression must be used. We were only able to obtain non-functioning mutants of the protein when we attempted to biobrick Tse2 downstream from F2620, probably due to leaky expression of the Tse2 toxin from this promoter.

Source

Pseudomonas aeruginosa

References

Cell Host Microbe.2010 Jan 21;7(1):25-37 PMID: 20114026