Difference between revisions of "Part:BBa K305007"
 
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<partinfo>BBa_K305007 short</partinfo>
 
<partinfo>BBa_K305007 short</partinfo>
  
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Promotor for the surfactin regulatory gene. Isolated from B.subtilis using small area around identified sequence with PCR.  
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Promoter for the surfactin regulatory gene. Isolated from ''B.subtilis'' using a shortened region between the response element and the transcription start side. The promoter is activated by quorum sensing, a process which it influences itself, during the late exponential stage. It activates the formation of bifilm, sporulation and natural competence of ''B.subtilis''. Complete pathway to srfAA promoter linked to ComXPA signaling shown below.  
  
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<!-- Add more about the biology of this part here
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[[Image:Groningen-ComXPA-srfAA.png|500px|none]]
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===Usage and Biology===
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[http://2010.igem.org/Team:Groningen#/expression iGEM Groningen 10]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K305007 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Part Characterization===
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===Functional Parameters===
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<partinfo>BBa_K305007 parameters</partinfo>
 
<partinfo>BBa_K305007 parameters</partinfo>
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Promoter activity visualized by GFP expression using part <partinfo>BBa_E0240</partinfo> in liquid minimal medium. The growth curve is overlaid by the relative fluorescence intensity to indicate the timing of the expression. Measurements performed in a Tecan microplate fluorometer and the fluorescence normalized for OD.
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[[Image:SrfAA1prODvsGFP.png|400px|none]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K305007 SequenceAndFeatures</partinfo>

Latest revision as of 22:05, 7 November 2010

srfA promoter, short variant

Promoter for the surfactin regulatory gene. Isolated from B.subtilis using a shortened region between the response element and the transcription start side. The promoter is activated by quorum sensing, a process which it influences itself, during the late exponential stage. It activates the formation of bifilm, sporulation and natural competence of B.subtilis. Complete pathway to srfAA promoter linked to ComXPA signaling shown below.

Groningen-ComXPA-srfAA.png

[http://2010.igem.org/Team:Groningen#/expression iGEM Groningen 10]


Part Characterization

Promoter activity visualized by GFP expression using part BBa_E0240 in liquid minimal medium. The growth curve is overlaid by the relative fluorescence intensity to indicate the timing of the expression. Measurements performed in a Tecan microplate fluorometer and the fluorescence normalized for OD.

SrfAA1prODvsGFP.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]