Difference between revisions of "Part:BBa K320002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The sequence was artificially made from two primers containing the ''E. coli'' consensus sequences for promoter and RBS with an appropriate spacing. | |
− | === | + | ===References=== |
− | + | # [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis] | |
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Latest revision as of 12:33, 27 October 2010
Strong promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was artificially made from two primers containing the E. coli consensus sequences for promoter and RBS with an appropriate spacing.
References
- [http://mic.sgmjournals.org/cgi/reprint/132/2/565.pdf Activity of two strong promoters cloned into Bacillus subtilis]