Difference between revisions of "Part:BBa K374006:Design"

(→‎Design Notes)
 
(4 intermediate revisions by 2 users not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Over expression of N-antiterminator leads to loss of specificity for ''nut''-sites.
+
This parts consist only of the coding sequence of lambda phage N protein.
  
 
===Source===
 
===Source===
  
−
N gene from the lambda phage from E.coli.
+
The gene has been taken out of the Lambda genome from N gene from the lambda phage from ''E. coli'' EMG2.
  
 
===References===
 
===References===
 +
* [1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.
 +
* [2] Franklin. Naomi. C., Doelling. Jed H. (1988). "Overexpression of N Antitermination Proteins of Bacteriophages lambda, 21 and P22: loss of protein specificity" Journal of Bacteriology.

Latest revision as of 00:55, 28 October 2010

Lambda N anti-terminator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This parts consist only of the coding sequence of lambda phage N protein.

Source

The gene has been taken out of the Lambda genome from N gene from the lambda phage from E. coli EMG2.

References

  • [1] Nudler, E. and Gottesman, M.E (2002). Transcription termination and anti-termination in E. coli. Genes to cells 7: 755-768.
  • [2] Franklin. Naomi. C., Doelling. Jed H. (1988). "Overexpression of N Antitermination Proteins of Bacteriophages lambda, 21 and P22: loss of protein specificity" Journal of Bacteriology.