Difference between revisions of "Part:BBa K389403"
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<partinfo>BBa_K389403 short</partinfo> | <partinfo>BBa_K389403 short</partinfo> | ||
− | This is a modified <partinfo>BBa_I746390</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4. | + | This is a modified <partinfo>BBa_I746390</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10[luc2] vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 30 - 35 fold compared to a system without sensitivity tuner ([http://2007.igem.org/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]). |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | For an aplication of this part look for BioBrick <partinfo>BBa_K389423</partinfo>. | ||
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Latest revision as of 21:33, 27 October 2010
Sensitivity tuner + luciferase
This is a modified BBa_I746390 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10[luc2] vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. The signal is supposed to be amplified 30 - 35 fold compared to a system without sensitivity tuner ([http://2007.igem.org/Cambridge/Amplifier_project#Results Cambridge, iGEM 2007, amplifier project]).
Usage and Biology
For an aplication of this part look for BioBrick BBa_K389423.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1322
Illegal NgoMIV site found at 2666
Illegal NgoMIV site found at 2687
Illegal AgeI site found at 845
Illegal AgeI site found at 957
Illegal AgeI site found at 1159
Illegal AgeI site found at 2390 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2572