Difference between revisions of "Part:BBa K404234"
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404234 short</partinfo>
 
<partinfo>BBa_K404234 short</partinfo>
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|-
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|'''Submitted by'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
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|}
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<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="left"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404234 (AAV2)-Rep-VP123_p5-TATAless (ViralBrick-587KO-Z34C-Spacer)]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_K404234 BBa_K404234]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|
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|-
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|'''Submitted by'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
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|}
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<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
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<br><b>The Z34C antibody binding motif including a knockout of the natural HSPG tropism & Spacer, inserted into the 587 loop of [[Part:BBa_K404002|[AAV2]-Rep-VP123_p5-TATAless]]</b>
  
 
[[Image:Freiburg10_Rep-VP123_P5-TATA-less 587KO-Z34C-Spacer.png|thumb|center|480px]]<br>
 
[[Image:Freiburg10_Rep-VP123_P5-TATA-less 587KO-Z34C-Spacer.png|thumb|center|480px]]<br>
  
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<!-- Add more about the biology of this part here
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The AAV capsid consists of 60 capsid protein subunits. The three cap proteins VP1, VP2, and VP3 are encoded in an overlapping reading frame. Arranged in a stoichiometric ratio of 1:1:10, they form an icosahedral symmetry. The mRNA encoding for the cap proteins is transcribed from p40 and alternative spliced to minor and major products. Alternative splicing and translation initiation of VP2 at a nonconventional ACG initiation codon promote the expression of VP1, VP2 and VP3. The VP proteins share a common C terminus and stop codon, but begin with a different start codon. The N termini of VP1 and VP2 play important roles in infection and contain motifs that are highly homologous to the phospholipase A2 (PLA2) domain and nuclear localization signals (BR)(+).
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<br />
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<br>
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===Usage and Biology===
 
===Usage and Biology===
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<html>
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<div style="width:965px; height:400px; ">
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<div style="float:left; width:460px; height:auto;  margin: 0px 5px 0px 5px; text-align:justify;">
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The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of  <a href=http://www.ncbi.nlm.nih.gov/protein/153106>Staphylococcal Protein A</a>. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.
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</div>
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<div style="float:right; width:480px; height:auto; "><img src="https://static.igem.org/mediawiki/parts/9/98/Freiburg10_ViralBrick_motif_Z34C.png" width="460"
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height="auto"/></div></div>
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</html>
  
 
<!-- -->
 
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Latest revision as of 02:59, 28 October 2010

[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-Z34C-Spacer) |- |Submitted by |[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] |}












(AAV2)-Rep-VP123_p5-TATAless (ViralBrick-587KO-Z34C-Spacer)
BioBrick Nr. BBa_K404234
RFC standard RFC 25
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]















The Z34C antibody binding motif including a knockout of the natural HSPG tropism & Spacer, inserted into the 587 loop of [AAV2]-Rep-VP123_p5-TATAless

Freiburg10 Rep-VP123 P5-TATA-less 587KO-Z34C-Spacer.png

The AAV capsid consists of 60 capsid protein subunits. The three cap proteins VP1, VP2, and VP3 are encoded in an overlapping reading frame. Arranged in a stoichiometric ratio of 1:1:10, they form an icosahedral symmetry. The mRNA encoding for the cap proteins is transcribed from p40 and alternative spliced to minor and major products. Alternative splicing and translation initiation of VP2 at a nonconventional ACG initiation codon promote the expression of VP1, VP2 and VP3. The VP proteins share a common C terminus and stop codon, but begin with a different start codon. The N termini of VP1 and VP2 play important roles in infection and contain motifs that are highly homologous to the phospholipase A2 (PLA2) domain and nuclear localization signals (BR)(+).

Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3611
    Illegal XhoI site found at 1913
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4254
    Illegal BsaI site found at 4436
    Illegal BsaI site found at 4473
    Illegal SapI site found at 3048