Difference between revisions of "Part:BBa K346034"

 
 
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<partinfo>BBa_K346034 short</partinfo>
 
<partinfo>BBa_K346034 short</partinfo>
  
This part is the combination of a T7promoter(BBa_I719005),RBS(B0034)and Lpp-OmpA+MBP(surface display of mercury binding peptide)
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This part is the combination of a T7promoter(BBa_I719005),RBS(B0034)and Lpp-OmpA+MBP(surface display of mercury binding peptide).It functions as the surface display part of MBP in our project.
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== Description ==
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It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts.
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Lpp-OmpA-mbp is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP). The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.
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        [[Image:Lpp-OmpA-MBP.jpg]]
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Figure 1: The structure of lpp-OmpA-MBP. This part can deliver the MBP to the surface and MBP will bind mercury more efficient and effective.
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[[Image:Exef df.jpg]]
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Figure 2: The expression of lpp-OmpA-MBP in the surface of our bacterial. We can see a clear band about 27KD in both SDS-PAGE and Western blotting.
  
 
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Latest revision as of 08:02, 26 October 2010

T7p(BBa_I719005)+RBS(B0034)+Lpp-OmpA+MBP(surface display of mercury binding peptide)

This part is the combination of a T7promoter(BBa_I719005),RBS(B0034)and Lpp-OmpA+MBP(surface display of mercury binding peptide).It functions as the surface display part of MBP in our project.


Description

It is highly expected that when fused with N-terminal fragment of Outer membrane protein A (OmpA), the mercury metal binding peptide(MBP)will be translocated and displayed on the surface of bacteria, which will contribute a lot to increase the mental binding capacity when combined will other collection parts.

Lpp-OmpA-mbp is designed as a fusion protein consisting of the signal sequence and first 9 amino acid of Lpp, residue 46~159 of OmpA and the metal binding peptide(MBP). The signal peptide of the N-termini of this fusion protein targets the protein on the membrane while the trans-membrane domain of Ompa serves as an anchor. MBP is on the externally exposed loops of OmpA, which can be anchored to the outer membrane.

       Lpp-OmpA-MBP.jpg

Figure 1: The structure of lpp-OmpA-MBP. This part can deliver the MBP to the surface and MBP will bind mercury more efficient and effective.

Exef df.jpg

Figure 2: The expression of lpp-OmpA-MBP in the surface of our bacterial. We can see a clear band about 27KD in both SDS-PAGE and Western blotting.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 660
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]