Difference between revisions of "Part:BBa K329000"
 
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Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Medium RBS upstream (<partinfo>BBa_B0032</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth.  
 
Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Medium RBS upstream (<partinfo>BBa_B0032</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth.  
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<br>We have designed several translational units for TetA(C) (see <partinfo>BBa_K329003</partinfo>, <partinfo>BBa_K329004</partinfo>)
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<br>We have designed several translational units for TetA(C) (see <partinfo>BBa_K329003</partinfo>, <partinfo>BBa_K329004</partinfo>).
  
 
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Latest revision as of 16:33, 12 October 2010

Medium RBS (B0032) - Tetracycline resistance protein TetA(C) (J31007)

Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Medium RBS upstream (BBa_B0032). However, overexpression of TetA(C) is detrimental to cell growth.
We have designed several translational units for TetA(C) (see BBa_K329003, BBa_K329004).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 335
    Illegal NgoMIV site found at 703
    Illegal NgoMIV site found at 863
  • 1000
    COMPATIBLE WITH RFC[1000]