Difference between revisions of "Part:BBa K329000"
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<partinfo>BBa_K329000 short</partinfo> | <partinfo>BBa_K329000 short</partinfo> | ||
| − | Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Medium RBS upstream (<partinfo>BBa_B0032</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. | + | Tetracycline resistance protein (TetA(C) inner-membrane-associated protein <partinfo>BBa_J31007</partinfo>) with a Medium RBS upstream (<partinfo>BBa_B0032</partinfo>). However, overexpression of TetA(C) is detrimental to cell growth. |
| + | <br>We have designed several translational units for TetA(C) (see <partinfo>BBa_K329003</partinfo>, <partinfo>BBa_K329004</partinfo>). | ||
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Latest revision as of 16:33, 12 October 2010
Medium RBS (B0032) - Tetracycline resistance protein TetA(C) (J31007)
Tetracycline resistance protein (TetA(C) inner-membrane-associated protein BBa_J31007) with a Medium RBS upstream (BBa_B0032). However, overexpression of TetA(C) is detrimental to cell growth.
We have designed several translational units for TetA(C) (see BBa_K329003, BBa_K329004).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 163
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 309
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 335
Illegal NgoMIV site found at 703
Illegal NgoMIV site found at 863 - 1000COMPATIBLE WITH RFC[1000]