Difference between revisions of "Part:BBa K424016:Design"
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| − | P. aeruginosa from INDICASAT and gene sequence reviewed in genbank. | + | ''P. aeruginosa'' from INDICASAT lab. and rhamnosiltransferase gene sequence reviewed in genbank. |
===References=== | ===References=== | ||
| + | Qinhong Wang, Xiangdong Fang, Baojun Bai, Xiaolin Liang, Patrick J. Shuler, William A. Goddard III, Yongchun Tang. 2007. [[Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery.]] Biotechnology and Bioengineering Volume 98, Issue 4, pages 842–853. | ||
Latest revision as of 22:08, 14 October 2010
Rhamnosiltransferase BioBrick (Rh1AB_BB)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 169
Illegal BamHI site found at 729
Illegal XhoI site found at 905
Illegal XhoI site found at 2191 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1084
Illegal NgoMIV site found at 1805
Illegal NgoMIV site found at 1918 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 394
Illegal BsaI site found at 1434
Illegal BsaI.rc site found at 578
Design Notes
illegal restriction enzymes lead us to apply multiple site mutagenesis technique.
Source
P. aeruginosa from INDICASAT lab. and rhamnosiltransferase gene sequence reviewed in genbank.
References
Qinhong Wang, Xiangdong Fang, Baojun Bai, Xiaolin Liang, Patrick J. Shuler, William A. Goddard III, Yongchun Tang. 2007. Engineering Bacteria for Production of Rhamnolipid as an Agent for Enhanced Oil Recovery. Biotechnology and Bioengineering Volume 98, Issue 4, pages 842–853.