Difference between revisions of "Part:BBa K389413"

 
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<partinfo>BBa_K389413 short</partinfo>
 
<partinfo>BBa_K389413 short</partinfo>
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This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
 
This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
  
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By modifying the Sensitivity Tuner the RBS from the phage activator from the original Sensitivity Tuner was  removed. New RBS comes with Virb-promotor element [https://parts.igem.org/cgi/sequencing/one_blast.cgi?id=7888 K389003].
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===Usage and Biology===
 
===Usage and Biology===
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For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389423</partinfo>
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Latest revision as of 21:50, 27 October 2010

vir promoter + sensitivity tuner + luciferase

This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.

By modifying the Sensitivity Tuner the RBS from the phage activator from the original Sensitivity Tuner was removed. New RBS comes with Virb-promotor element K389003.


Usage and Biology

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389423


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2455
    Illegal NgoMIV site found at 3799
    Illegal NgoMIV site found at 3820
    Illegal AgeI site found at 1978
    Illegal AgeI site found at 2090
    Illegal AgeI site found at 2292
    Illegal AgeI site found at 3523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3705