Difference between revisions of "Part:BBa K371018"

 
 
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<partinfo>BBa_K371018 short</partinfo>
 
<partinfo>BBa_K371018 short</partinfo>
  
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This composite consists of eight pdu bacterial microcompartment(BMC) structure gene following a natural lac promotor(BBa_E0010). These genes come from four basic parts,pduAB,pduJK,pduN and pduTUV, which consist of the natural RBS and gene separately.
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This composite part is RFP protein used for protein fusion according to BBF [RFC53][http://bbf.openwetware.org/RFC.html#BBF_RFC_53:_USTC_MetaPart_Assembly_Standard_--_Extending_RFC_10_to_Enable_Scarless_Protein_Fusion_with_Type_IIS_Restriction_Enzyme_EarI_and_SapI]. It origin from BioBrick BBa_E0043 with MPF and MSF at the two end of its sequence.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 10:49, 27 October 2010

MPF+RFP+MSF

This composite part is RFP protein used for protein fusion according to BBF [RFC53][http://bbf.openwetware.org/RFC.html#BBF_RFC_53:_USTC_MetaPart_Assembly_Standard_--_Extending_RFC_10_to_Enable_Scarless_Protein_Fusion_with_Type_IIS_Restriction_Enzyme_EarI_and_SapI]. It origin from BioBrick BBa_E0043 with MPF and MSF at the two end of its sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 565
    Illegal AgeI site found at 677
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3