Difference between revisions of "Part:BBa K330002"

 
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<partinfo>BBa_K330002 short</partinfo>
 
<partinfo>BBa_K330002 short</partinfo>
  
The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme from Escherichia coli.
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The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme originated from Escherichia coli.
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Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay,  4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl &#946;-D-glucuronide for spectrophotometric assay.
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THE CHARACTERIZATION RESULTS OF BBa_K330002 IS UNDER 'EXPERIENCE' PAGE (https://parts.igem.org/Part:BBa_K330002:Experience).
  
Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacteria. There are three often used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays vary (5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay,  4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl &#946;-D-glucuronide for spectrophotometric assay).
 
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 04:37, 7 November 2010

gusA reporter gene

The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme originated from Escherichia coli.

Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay.

THE CHARACTERIZATION RESULTS OF BBa_K330002 IS UNDER 'EXPERIENCE' PAGE (https://parts.igem.org/Part:BBa_K330002:Experience).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]