Difference between revisions of "Part:BBa K364319:Design"

 
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===Design Notes===
 
===Design Notes===
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The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25
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The construction of the DBD and pSB1C3 backbone have been done with RFC 25.
  
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===Source===
  
 +
Yeast transcription activator protein. Arteficially synthetized then placed into pSB1C3 carrier vector.
  
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===Source===
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===References===
 +
Ana Traven, Branka Jelicic & Mary Sopta. "Yeast Gal4: a transcriptional paradigm revisited". EMBO reports (2006) 7, 496 - 499.
  
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Regulatory protein
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Galen A. Collins, J. Russell Lipford, Raymond J. Deshaies  &  William P. Tansey. "Gal4 turnover and transcription activation". Nature 461, E7 (8 October 2009).
  
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===References===
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K. K. Rodgers and J. E. Coleman. "DNA binding and bending by the transcription factors GAL4(62*) and GAL4(149*)".
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Protein Sci. 1994 April; 3(4): 608–619.

Latest revision as of 21:51, 25 October 2010

Gal4 DBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


Design Notes

The construction of the DBD and pSB1C3 backbone have been done with RFC 25.

Source

Yeast transcription activator protein. Arteficially synthetized then placed into pSB1C3 carrier vector.

References

Ana Traven, Branka Jelicic & Mary Sopta. "Yeast Gal4: a transcriptional paradigm revisited". EMBO reports (2006) 7, 496 - 499.

Galen A. Collins, J. Russell Lipford, Raymond J. Deshaies & William P. Tansey. "Gal4 turnover and transcription activation". Nature 461, E7 (8 October 2009).

K. K. Rodgers and J. E. Coleman. "DNA binding and bending by the transcription factors GAL4(62*) and GAL4(149*)". Protein Sci. 1994 April; 3(4): 608–619.