Difference between revisions of "Part:BBa K411100:Design"

 
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<partinfo>BBa_K411100 short</partinfo>
 
<partinfo>BBa_K411100 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Mutated 2 PstI cutting sites from ctgcag to ctccag
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Mutated two PstI cutting sites at positions 223 and 391 from ctgcag to ctccag. <!-- See our wiki for the details of the PCR primers used to do the point mutation -->
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===Source===
 
===Source===
  
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''Mus Musculus''
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[http://www.ncbi.nlm.nih.gov/nuccore/NM_144958 eIF4A] from ''Mus Musculus''
  
 
===References===
 
===References===

Latest revision as of 07:13, 7 October 2010

Eukaryote Initiation Factor 4A (eIF4A) from Mus Musculus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 240
    Illegal BglII site found at 536
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 972
    Illegal BsaI.rc site found at 638


Design Notes

Mutated two PstI cutting sites at positions 223 and 391 from ctgcag to ctccag.

Source

[http://www.ncbi.nlm.nih.gov/nuccore/NM_144958 eIF4A] from Mus Musculus

References