Difference between revisions of "Part:BBa K395704:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K395704 short</partinfo> | <partinfo>BBa_K395704 short</partinfo> | ||
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・crtZ is under Arabinose inducible promoter. | ・crtZ is under Arabinose inducible promoter. | ||
− | ・crtZ is located at | + | ・crtZ is located at upstream of crtEBIY because the suffix of BBa_K274210(crtEBIY) is out of order and it is impossible to bring it at downstream. |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Nishizaki T, et al. Metabolic engineering of carotenoid biosynthesis in ''Escherichia coli'' by ordered gene assembly in Bacillus subtilis. Appl Environ Microbiol. 2007 Feb | ||
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+ | Seon-Kang C, et al. Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ, | ||
+ | and P450 in astaxanthin biosynthetic pathway and adonirubin | ||
+ | production by gene combination in ''Escherichia coli''. Appl Microbiol Biotechnol (2006) 72 | ||
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+ | Arunkumar K, et al. Structure of a carotenoid gene cluster from Pantoea sp. strain C1B1Y and characterization of β-carotene hydroxylase(crtZ)gene by functional complementation in ''Escherichia coli''. Carotenoid Science Vol.11 (2007) |
Latest revision as of 11:13, 21 October 2010
zeaxanthin synthesis operon (pBad+rbs+crtZ+plac+rbs +crtEBIY )
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 3810 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3346
Illegal NgoMIV site found at 3476
Illegal AgeI site found at 979
Illegal AgeI site found at 2561 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
・crtZ is under Arabinose inducible promoter.
・crtZ is located at upstream of crtEBIY because the suffix of BBa_K274210(crtEBIY) is out of order and it is impossible to bring it at downstream.
Source
Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.
This Composite Biobrick is created by standard assembly of Part BBa_K395702, Part BBa_K274210.
References
Nishizaki T, et al. Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis. Appl Environ Microbiol. 2007 Feb
Seon-Kang C, et al. Characterization of bacterial β-carotene 3,3′-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli. Appl Microbiol Biotechnol (2006) 72
Arunkumar K, et al. Structure of a carotenoid gene cluster from Pantoea sp. strain C1B1Y and characterization of β-carotene hydroxylase(crtZ)gene by functional complementation in Escherichia coli. Carotenoid Science Vol.11 (2007)