Difference between revisions of "Part:BBa K395702"
(4 intermediate revisions by 2 users not shown) | |||
Line 3: | Line 3: | ||
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. | This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. | ||
− | It is necessary to add either beta-carotene or the synthesis operon. | + | |
+ | It is necessary to add either beta-carotene or the synthesis operon.this part applies to ② and ④ | ||
+ | |||
+ | [[Image:Tokyotech_registry_zeaxanthin.jpg|center|650px|thumb|Fig.2 zeaxanthin pellet and plate, TLC<br>These works are done by Yumiko Kinoshita]] | ||
+ | |||
+ | These parts contain ''crtZ'' (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in ''E. coli'' strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information]) | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 23:48, 27 October 2010
rbs+crtZ under pBad promoter for double plasmids
This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate.
It is necessary to add either beta-carotene or the synthesis operon.this part applies to ② and ④
These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961