Difference between revisions of "Part:BBa K395600:Design"
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| − | + | The gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala). | |
===References=== | ===References=== | ||
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| + | Edwige J. F. Souleyre et al. An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor, FEBS Journal, 272, 3132–3144 (2005) | ||
Latest revision as of 19:50, 27 October 2010
Apple fragrance enzyme (MpAAT1) generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1666
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 195
Illegal BamHI site found at 1635
Illegal XhoI site found at 1675 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 205
Illegal AgeI site found at 1419 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag
Source
The gene MpAAT1 was isolated from Malus pumila (cv. Royal Gala).
References
Edwige J. F. Souleyre et al. An alcohol acyl transferase from apple (cv. Royal Gala), MpAAT1, produces esters involved in apple fruit flavor, FEBS Journal, 272, 3132–3144 (2005)