Difference between revisions of "Part:BBa K352003"

Latest revision as of 15:18, 27 October 2010

pCooM Promoter from Rhodospirillum rubrum

PcooF and PcooM, the two CooA-regulated R. rubrum promoters, contain 2-fold symmetric DNA sequences which are CooA-binding sites and are similar to the CRP consensus sequence. This similarity corralates with the tantamount helix-turn-helix motifs CooA and CRP. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo.

The two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ, is the reason for the CO-dependent anaerobic growth of Rhodospirillum rubrum and enable the bacteria to oxidize CO . The coo regulon has three main products which are listed as O2-sensitive CO dehydrogenase (CooS), CooS-associated Fe-S protein (CooF), CO-tolerant hydrogenase (CooH). CooA is responsible for the expression of the genes of these proteins and activates by sensing CO in anaerobic environments. Because of the lack of active CooA, CO-independent basal level transcription of PcooF is not detectable.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K352003 short</partinfo>
-The two CooA-regulated R. rubrum promoters, PcooF and PcooM, contain 2-fold symmetric DNA sequences that serve as CooA-binding sites and are similar to the CRP consensus sequence. This is consistent with the similarity between CooA and CRP in their helix-turn-helix motifs. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo.
+PcooF and PcooM, the two CooA-regulated R. rubrum promoters, contain 2-fold symmetric DNA sequences which are CooA-binding sites and are similar to the CRP consensus sequence. This similarity corralates with the tantamount helix-turn-helix motifs CooA and CRP. The CooA binding sites lie at the 243.5 and 238.5 positions relative to the transcription start sites in PcooF and PcooM, respectively, overlapping with the 235 region. This overlap suggests that both CooA-regulated promoters are analogous to class II CRP-dependent promoters. We chose PcooF for this study because it is the stronger promoter based on the amount of primer extension product and level of coo-encoded proteins synthesized in vivo.
-The CO-dependent anaerobic growth of Rhodospirillum rubrum relies on a CO oxidation system encoded by two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ. The key products of the coo regulon are an O2- sensitive CO dehydrogenase (CooS), a CooS-associated Fe-S protein (CooF), and a CO-tolerant hydrogenase (CooH), and the expression of the genes depends upon the activity of the CooA protein, which senses CO under anaerobic conditions.
+The two CO regulated transcriptional units, cooMKLXUH and cooFSCTJ, is the reason for the CO-dependent anaerobic growth of Rhodospirillum rubrum and enable the bacteria to oxidize CO . The coo regulon has three main products which are listed as O2-sensitive CO dehydrogenase (CooS), CooS-associated Fe-S protein (CooF), CO-tolerant hydrogenase (CooH). CooA is responsible for the expression of the genes of these proteins and activates by sensing CO in anaerobic environments. Because of the lack of active CooA, CO-independent basal level transcription of PcooF is not detectable.
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